Paintings in fictional horror are fairly numerous such as Oscar Wilde’s The Picture of Dorian Gray, Nathaniel Hawthorne’s Edward Randolph's Portrait; Montague Rhodes James’s excellent The Mezzotint; and H. P. Lovecraft’s horror-inducing Pickman's Model; to name a few. Recently posted to this canon of haunted imagery, my own The Painted Smile, part of the new Dancing in Darkness series.
Out of the pages of fiction, there exist many stories of real-life stories of haunted paintings and hoaxes. Among my favorites: http://whofortedblog.com/2013/05/27/mail-bag-update-spooky-anguished-man-haunted-painting/
Hoax...or not? http://www.castleofspirits.com/hauntedpainting.html